Development and Validation of a Dual-Wavelength RP-HPLC-DAD Method for the Determination of Bisphenol A in Bottled Drinking Water

Zahid H. Shar; Pawan Kumar; Faisal Ali; Muhammad Younis Talpur; Abdur Rehman; Muqadas Abbasi; Anoosha Maryam; Gul Afshan Soomro; Muhammad Kashif Channa

doi:10.56810/jpbm.004.02.0128

Authors

Zahid H. Shar Dr. M.A. Kazi Institute of Chemistry, University of Sindh, Jamshoro
Pawan Kumar Dr. M. A. Kazi Institute of Chemistry, University of Sindh Jamshoro
Faisal Ali Department of Industrial Chemistry, NEDUET, Karachi
Muhammad Younis Talpur Dr. M. A. Kazi Institute of Chemistry, University of Sindh Jamshoro
Abdur Rehman National Reference Laboratory for the Department of Plant Protection (NRL-DPP), International Center for Chemical and Biological Sciences, University of Karachi
Muqadas Abbasi Dr. M. A. Kazi Institute of Chemistry, University of Sindh Jamshoro
Anoosha Maryam Dr. M. A. Kazi Institute of Chemistry, University of Sindh Jamshoro
Gul Afshan Soomro Institute of Chemistry, Shah Abdul Latif University Khairpur
Muhammad Kashif Channa Institute of Chemistry, Shah Abdul Latif University Khairpur

DOI:

https://doi.org/10.56810/jpbm.004.02.0128

Keywords:

Bisphenol A, Water contamination HPLC-DAD, Endocrine disruptor

Abstract

Contamination of bottled water with bisphenol A cause serious health issues and needs continuous monitoring. For this purpose, a rapid and cost-effective method for BPA has been validate by using dual wavelength high-performance liquid chromatography with diode array detection (HPLC-DAD) in bottled drinking water. Concentration of BPA was measured at two wavelength (205 nm and 224 nm) after separation by using Hypersil ODS C18 column (5 μm, 250 × 3.0 mm) using water/acetonitrile (50:50, v/v) mobile phase. Flow rate was maintained at 0.5 mL/min and column temperature at 30°C. the method showed Excellent linearity at both wavelength (R² = 0.999 at 205 nm; R² = 0.9996 at 224 nm) over a concentration range of 2.5–50 μg/L. The limit of detection (LOD) and limit of quantification (LOQ) were 1.66 μg/L and 5.03 μg/L, respectively. Accuracy and Precision was found to be 92–95% and 92–93%, 2.75% and 4.1% respectively at both wavelengths. The method offers a reliable, sensitive, and economical analytical tool for routine monitoring of BPA contamination in drinking water bottles.