Evaluation of Anti-Inflammatory Potential of Morin Anhydrous using Acute and Chronic Dermatitis Mice Models

Abstract

A complicated biological process, inflammation is triggered by exposure to toxins, chemicals, infections, ad injury. Consumption of several non-steroidal anti-inflammatory drugs (NSAIDs) and glucocorticosteroids, commercially available, provide the treatment for inflammation results in various side effects like itching, rash, photosensitivity and angioedema. This highlights requirement for developing the novel anti-inflammatory agent that can help to cure inflammation with fewer adverse effects. Flavonoids and other natural substances have shown positive effects on dermatitis. Natural bioflavonoid Morin (3,5,7,2′,4′-pentahydroxyflavone), which is a key ingredient in medicinal herbs, was first isolated from plants in the Moraceae family, including figs (Chlorophora tinctoria) and almonds (Prunus dulcis). Morin possesses anti-inflammatory and antioxidant properties but its connection to dermatitis has’nt yet been investigated.  Goal of this study was to examine in vitro antioxidant and in vivo anti-inflammatory properties of Morin using different models. Morin’s antioxidant activity was assessed using in vitro assays, namely 2, 2-diphenyl-1-picrylhydrazyl (DPPH), 2, 2-azino-bis-3-3-thylbenzthiazoline-6-sulphonic acid (ABTS), and lipid peroxidation (LPO). The findings demonstrated that Morin is the potent antioxidant agent, depending on the dose and its significance. DPPH (23.6 µM), ABTS (13.86 µM), and LPO (91.81 µM) are the IC⁵⁰ values. Additionally, acute skin inflammatory mice model induced by TPA and chronic skin inflammatory mice model induced by oxazolone were used to investigate Morin's in vivo anti-inflammatory properties. Two criteria, namely edema weight and edema volume, were used to assess the degree of edema. According to the findings, edema weight and volume were reduced by 50% and 80%, respectively, in acute inflammatory model of TPA, whereas both were reduced by 60% in the chronic inflammatory model of oxazolone. In summary, this study illustrates Morin's anti-inflammatory and antioxidant properties. Consequently, it is possibly referred to be a novel therapeutic approach for treating variety of inflammatory skin conditions.